Difference between revisions of "HughesLab:Protocols"

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{{HughesLab}}
 
{{HughesLab}}
 
 
== Fly Behavior and Genetics ==
 
 
 
 
 
 
  
  
 
== Molecular Biology ==
 
== Molecular Biology ==
 
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
 
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
 
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* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
 +
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
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* RNA prep using Trizol and Qiagen RNeasy columns: [[Image:RNA_prep_using_Trizol.docx]]
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<br>
  
 
== Next-generation sequencing ==
 
== Next-generation sequencing ==
 
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
 
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
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<br>
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== Fly Behavior and Genetics ==
 +
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
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* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]]
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<br>
  
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== Analytics ==
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* Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]]
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<br>
  
  
 
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[[Image:UMSL_logo.jpg|left|100px]]<br>
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[[Image:UMSL_logo.jpg|left|100px]]
 
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Hughes Lab<br>
 
[http://www.umsl.edu/~biology/ Department of Biology]<br>
 
[http://www.umsl.edu/~biology/ Department of Biology]<br>
 
University of Missouri, St. Louis<br>
 
University of Missouri, St. Louis<br>
 
(Starting in August, 2013)
 
(Starting in August, 2013)

Revision as of 07:42, 13 February 2013

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Molecular Biology


Next-generation sequencing


Fly Behavior and Genetics


Analytics




UMSL logo.jpg

Hughes Lab
Department of Biology
University of Missouri, St. Louis
(Starting in August, 2013)