Difference between revisions of "HughesLab:Protocols"

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== Fly Behavior and Genetics ==
 
  
  
 
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* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
 
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
 
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
 
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]
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* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
  
 
== Next-generation sequencing ==
 
== Next-generation sequencing ==
 
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
 
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
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== Fly Behavior and Genetics ==
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Revision as of 09:00, 8 January 2013

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Hughes Lab
Department of Biology
University of Missouri, St. Louis
(Starting in August, 2013)