Difference between revisions of "HughesLab:Protocols"

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(Fly Behavior and Genetics)
 
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== Molecular Biology ==
 
== Molecular Biology ==
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
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* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:RNA_prep_using_Trizol.docx]]<br>
 
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
 
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
 
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
 
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
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* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
 
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
 
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== Fly Behavior and Genetics ==
 
== Fly Behavior and Genetics ==
 
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
 
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
* Vials for DAM analysis:  
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* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]]
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* Fly food protocol: [[Image:Fly_food.docx]]
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== Analytics ==
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* Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]]
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* RUM (RNA-Seq Unified Mapper) guide: [[Image:RUM guide.pdf]]
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* Basic linux commands: [[Image:linux commands.pdf]]
 
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== Analytics ==
 
* Human temperature record analysis:
 
 
 
  
 
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[[Image:UMSL_logo.jpg|left|100px]]
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[[Image:WashU_Logo.png|left|150px]]
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Hughes Lab<br>
 
Hughes Lab<br>
[http://www.umsl.edu/~biology/ Department of Biology]<br>
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[https://pulmonary.wustl.edu/ Pulmonary and Critical Care Medicine]<br>
University of Missouri, St. Louis<br>
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Washington University School of Medicine
(Starting in August, 2013)
 

Latest revision as of 09:00, 5 July 2017

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Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine