Hop DNA Isolation: Difference between revisions
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Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM | Buffer: 100mL: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/mL of CTAB and 20muL/mL 2-mercaptoethanol. |
Revision as of 13:32, 5 March 2007
Hop DNA Extraction Protocol
- Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
- Assume 90% of mass is water weight.
- Add
Buffer: 100mL: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/mL of CTAB and 20muL/mL 2-mercaptoethanol.