Hop DNA Isolation: Difference between revisions
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(New page: ==Hop DNA Extraction Protocol== Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM) |
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==Hop DNA Extraction Protocol== | ==Hop DNA Extraction Protocol== | ||
#Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit). | |||
#Assume 90% of mass is water weight. | |||
#Add | |||
Revision as of 13:18, 5 March 2007
Hop DNA Extraction Protocol
- Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
- Assume 90% of mass is water weight.
- Add
Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM
