Difference between revisions of "Hop DNA Isolation"

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(New page: ==Hop DNA Extraction Protocol== Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM)
 
(Hop DNA Extraction Protocol)
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==Hop DNA Extraction Protocol==
 
==Hop DNA Extraction Protocol==
 
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#Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
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#Assume 90% of mass is water weight.
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#Add
  
  

Revision as of 12:18, 5 March 2007

Hop DNA Extraction Protocol

  1. Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
  2. Assume 90% of mass is water weight.
  3. Add



Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM