Difference between revisions of "HighPoint/CannonLab:Buffer and Osmolyte Solutions"

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(New page: {{CannonLabMenu}} ==Preparing Buffers== ===Stock Solutions=== # Prepare stock solutions of buffer components (typically 1 M) # Rinse ''clean'' storage bottle and filter flask an ''extra...)
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Preparing Buffers

Stock Solutions

  1. Prepare stock solutions of buffer components (typically 1 M)
  2. Rinse clean storage bottle and filter flask an extra time
    • Use type I, 18 MΩ purified water
    • Drain and use your best aseptic technique from this point on
  3. Filter stock solutions through 0.2 μm filter
  4. Store in bottle for future use
    • Keep the bottle closed as much as possible
    • Always wear gloves and use clean instruments for removing aliquots
    • This will extend the life of your stock solution before bacteria begin to grow in it

Filtering

General rules for solution prep

Adjusting pH

Useful Recipes

1 M K2HPO4

1 M KH2PO4

1 M NaAcetate

1 M Acetic Acid

pH 7 buffer, 10 mM Phosphate, 50 mM NaCl