Difference between revisions of "Haynes Lab:Notebook:Shared/Cloning Bootcamp"

From OpenWetWare
Jump to: navigation, search
(2/5/2014)
Line 16: Line 16:
  
 
The objective of this project is to successfully replicate delicate DNA. In the first session of this cloning boot camp we manipulate bacteria to take plasmid DNA in order to create large amounts of the cell.
 
The objective of this project is to successfully replicate delicate DNA. In the first session of this cloning boot camp we manipulate bacteria to take plasmid DNA in order to create large amounts of the cell.
 
=2/5/2014=
 
 
 
'''Bootcamp 1st session'''
 
 
Transforming Bacteria with Plasmid DNA
 
Miriam Acosta
 
February 5, 2014
 
 
# List items
 
##Plasmid: KAH187
 
##BioBrick Part Number: BBa-J176094
 
##Total Length: 2275 bp
 
##Vector Backbone:
 
##Antibiotic Resistance Gene: Ampicillin
 
##Length of of Vector Backbone: 4200bp
 
 
 
#Samples for Transformations
 
##Sample 1:  Plasmid DNA - [KAH182]
 
## Sample 2:  Negative Control (water)
 

Revision as of 13:26, 12 February 2014

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">06/25/2013,06/28/2013,02/05/2014,01/27/2015,01/29/2015,02/03/2015,02/06/2015,02/24/2015,02/25/2015,02/26/2015,03/05/2015,03/15/2015,03/24/2015,03/26/2015,04/27/2015,04/28/2015,04/29/2015,05/13/2015,05/14/2015,07/27/2015,07/28/2015,07/29/2015,11/16/2015,11/18/2015,11/20/2015,01/15/2016</div><div style="display:none;" id="page">Haynes Lab:Notebook:Shared/Cloning Bootcamp</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Project Description/Abstract

The objective of this project is to successfully replicate delicate DNA. In the first session of this cloning boot camp we manipulate bacteria to take plasmid DNA in order to create large amounts of the cell.