Difference between revisions of "Haynes Lab:Notebook/TB Biosensor/2012/09/13"

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(Making crt Fusion Proteins by Gibson Assembly)
(Making crt Fusion Proteins by Gibson Assembly)
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* Ernesto PCR'd the overhangs onto the crt genes for Gibson assembly.  
 
* Ernesto PCR'd the overhangs onto the crt genes for Gibson assembly.  
  
[[https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdDhDR1hFUXlvSFZleGd2RWhrcW03QXc | Primer details]] with sequences
+
[https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdDhDR1hFUXlvSFZleGd2RWhrcW03QXc Primer details] with sequences
  
 
{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->

Revision as of 10:44, 14 September 2012

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Making crt Fusion Proteins by Gibson Assembly

  • Ernesto PCR'd the overhangs onto the crt genes for Gibson assembly.

Primer details with sequences

Final Construct Template Forward Primer Reverse Primer
crtE - PLFlex - X crtE P001 P010
X- PLFlex - crtE crtE P015 P002
crtI - PLFlex - X crtI P005 P012
X- PLFlex - crtI crtI P017 P006
crtB - PLFlex - X crtB P007 P014
X- PLFlex - crtB crtB P019 P008
crtE - PLFlex - crtB PLFlex P009 P020
crtE - PLFlex - crtI PLFlex P009 P018
crtI - PLFlex - crtE PLFlex P011 P016
crtI - PLFlex - crtB PLFlex P011 P020
crtB - PLFlex - crtE PLFlex P013 P016
crtB - PLFlex - crtI PLFlex P013 P018