Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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* Prepared rxn setup for plates
 
* Prepared rxn setup for plates
  
3x reaction:
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3x reaction: <br/>
Probe master mix, (7.5 x3) 22.5 uL
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Probe master mix, (7.5 x3) 22.5 uL <br/>
1:10 cDNA, (2.0 x3) 6.0 uL
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1:10 cDNA, (2.0 x3) 6.0 uL <br/>
dH2O, (0.2 x3) 0.6 uL
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dH2O, (0.2 x3) 0.6 uL <br/>
 
Total = (9.7 x3) 29.1
 
Total = (9.7 x3) 29.1
 
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<br/>
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<br/>
 
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
 
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
 
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*Note: To dilute cDNA add 10uL of cDNA to 90uL of water
 
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Latest revision as of 22:28, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).

  • Note: To dilute cDNA add 10uL of cDNA to 90uL of water