Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20

From OpenWetWare
Revision as of 10:28, 22 February 2013 by Caroline Hom (talk | contribs) (Assay Design)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).