Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/01/07: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Caroline Hom (talk | contribs) (Autocreate 2013/01/07 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==RNeasy Mini Kit== | ||
#Add 100μL of chloroform to each tube | |||
#Shake vigorously for 20 sec and let sit for 2 min | |||
#Centrifuge in cold room for 15 min @ 12,000G | |||
#Pipet off clear phase from organic phase into new tube (warning: do not get pink stuff in the tube!) | |||
#Add 70% ethanol into tube (1:1 ratio) | |||
#Transfer to collection tube (we have K562 1, K562 2, SK-N-SH 1, and SK-N-SH 2) | |||
#Spin tubes at top speed for 30 sec | |||
#Discard collected liquid and add 700μL of RW1 Buffer | |||
#Spin tubes at top speed for 30 sec | |||
#Put collection vial in a fresh collector tube and discard of the old one | |||
#Add 500μL of RBE Buffer | |||
#Spin tubes at top speed for 30 sec then discard of the liquid waste | |||
#Repeat the above two steps again | |||
#Discard of the waste and spin again | |||
#Transfer liquid to a fresh tube and add 30mL of RNase-Free H20 | |||
#Let tubes sit for 1 min | |||
#Spin tubes for 1 min | |||
#Store at -80°C | |||
Latest revision as of 22:21, 26 September 2017
Project name | Main project page Previous entry Next entry |
RNeasy Mini Kit
|