Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/18"

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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
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==Cells in Trizole==
* Insert content here...
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* Cells in trizole at 80°C
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'''K562'''
 +
#Expand cells to 10cm petri dishes for 10mL of cells
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#For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total)
 +
#Spin tubes at room temperature @ 3000 RPM for 5 min
 +
#Dispose of supernatant
 +
#Add 500μL of trizol to each pellet
 +
#Store @ -80°C
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'''SK-N-SH'''
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#From the 6 well plate aspirate off the growth media
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#Add 500μL of trizol directly to the 6 well plate
 +
#Let sit for 5 min @ room temperature under the hood
 +
#Use 100m0mL pipet to scrape and pipet up and down to break up cells
 +
#Transfer 500μL into each tube
  
  

Latest revision as of 21:21, 26 September 2017

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Cells in Trizole

  • Cells in trizole at 80°C

K562

  1. Expand cells to 10cm petri dishes for 10mL of cells
  2. For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total)
  3. Spin tubes at room temperature @ 3000 RPM for 5 min
  4. Dispose of supernatant
  5. Add 500μL of trizol to each pellet
  6. Store @ -80°C

SK-N-SH

  1. From the 6 well plate aspirate off the growth media
  2. Add 500μL of trizol directly to the 6 well plate
  3. Let sit for 5 min @ room temperature under the hood
  4. Use 100m0mL pipet to scrape and pipet up and down to break up cells
  5. Transfer 500μL into each tube