Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12

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Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)