Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12: Difference between revisions
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==Primer Prep== | ==Primer Prep== | ||
* See excel document for number correlation | * See excel document for number correlation | ||
#Add nm x 100 = | #Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer | ||
#Heat @ | #Heat @ 37°C for ~5min, vortex | ||
#In a fresh 1.5mL tube add | #In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution) | ||
'''Tube Numbering (label number on tube top and concentration of solution on side''' | '''Tube Numbering (label number on tube top and concentration of solution on side''' | ||
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] | [[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] | ||
Revision as of 01:31, 20 December 2012
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Primer Prep
Tube Numbering (label number on tube top and concentration of solution on side
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