Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12"

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(Autocreate 2012/12/12 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
 
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==Entry title==
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==Primer Prep==
* Insert content here...
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* See excel document for number correlation
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#Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
 +
#Heat @ 37C for ~5min, vortex
 +
#In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)
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Revision as of 00:12, 20 December 2012

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Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)