Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12"

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==Primer Prep==
 
==Primer Prep==
 
* See excel document for number correlation
 
* See excel document for number correlation
#Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
+
#Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
#Heat @ 37C for ~5min, vortex
+
#Heat @ 37°C for ~5min, vortex
#In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)
+
#In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)
 
+
'''Tube Numbering (label number on tube top and concentration of solution on side'''
 
+
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]]
  
 
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Revision as of 00:31, 20 December 2012

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Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37°C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)

Tube Numbering (label number on tube top and concentration of solution on side Screen Shot 2012-12-20 at 1.17.20 AM.png