Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2012/12/12 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==Primer Prep== | ||
* | * See excel document for number correlation | ||
#Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer | |||
#Heat @ 37°C for ~5min, vortex | |||
#In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution) | |||
'''Tube Numbering (label number on tube top and concentration of solution on side''' | |||
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] | |||
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Revision as of 01:31, 20 December 2012
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Primer Prep
Tube Numbering (label number on tube top and concentration of solution on side
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