Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12"

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(Autocreate 2012/12/12 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
 
(Primer Prep)
 
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==Entry title==
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==Primer Prep==
* Insert content here...
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* See excel document for number correlation
 
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#Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
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#Heat @ 37°C for ~5min, vortex
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#In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)
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'''Tube Numbering (label number on tube top and concentration of solution on side'''
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[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]]
  
 
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Latest revision as of 01:31, 20 December 2012

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Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37°C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)

Tube Numbering (label number on tube top and concentration of solution on side Screen Shot 2012-12-20 at 1.17.20 AM.png