Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/30: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2012/11/30 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==SK-N-SH Expansion== | ||
* | * 2 new 75cm flasks for SK-N-SH | ||
#1:5 passage (10mL fresh - 2mL cell, 8mL media) | |||
#1:4 passage (10mL fresh - +8mL fresh to re-suspend cells) | |||
'''Procedure''' | |||
#Aspirate off media (titlt) | |||
#Add 5mL of PBS to wash the cells | |||
#Aspirate off PBS | |||
#Add 2mL of trypsine (wash down walls) and let sit for 4-5 min | |||
#Knock on container (a sufficient amount) to suspend cells | |||
#Make the 2 75cm flasks as shown above, incubate | |||
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Revision as of 01:25, 20 December 2012
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SK-N-SH Expansion
Procedure
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