Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14"

From OpenWetWare
Jump to: navigation, search
(→‎Splitting Cells)
(fix raw html notebook nav)
 
(2 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
−
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Splitting Cells==
 
==Splitting Cells==
−
Seed Culture
+
'''Seed Culture'''
 
# 12-well plate
 
# 12-well plate
 
# 75mL flask
 
# 75mL flask
  
−
5X diluted cells
+
'''5X diluted cells'''
 +
<br/>
 
1. 25mL diluted cells  
 
1. 25mL diluted cells  
−
<br/>
 
 
*5mL cells + 20mL medium in a 50mL conical vial
 
*5mL cells + 20mL medium in a 50mL conical vial
 
*Pipette up and down
 
*Pipette up and down
 
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
 
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
 
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
 
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
−
 
−
 
−
 
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 22:16, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells
1. 25mL diluted cells

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2/2012/11/14&oldid=1011546"