Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Splitting Cells== | ==Splitting Cells== | ||
Seed Culture | '''Seed Culture''' | ||
# 12-well plate | # 12-well plate | ||
# 75mL flask | # 75mL flask | ||
5X diluted cells | '''5X diluted cells''' | ||
1. 25mL diluted cells | <br/> | ||
1. 25mL diluted cells | |||
*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down | ||
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:16, 26 September 2017
Project name | Main project page Previous entry Next entry |
Splitting CellsSeed Culture
5X diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" |