Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/10/29: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Caroline Hom (talk | contribs) (Autocreate 2012/10/29 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
Caroline Hom (talk | contribs) |
||
(One intermediate revision by the same user not shown) | |||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Expanding Cells== | ||
* | * Expand cell cultures: K-562, SK-N-SH | ||
---- | |||
'''Expand cultures 4x'''<br> | |||
* Checked cells under scope. Good amount of cell division | |||
* K-562 (suspension) | |||
** Split 10 mL culture into two (5 mL in 2 15 mL tubes) | |||
** Spun for 3 min, 1000 rpm, room temp | |||
** Resuspended each 5 mL pellet in 10 mL IMDM (10% FBS, 1% pen/strep) | |||
** Added 5 mL resuspension to 5mL medium in 75 cm flask (10 mL final volume) | |||
** Made 4 total 75 mL falsks | |||
* SK-N-SH | |||
** Aspirate old medium, wash with 2 mL 1xPBS; detach with 0.5 mL Trypsin media (3 min.); collect in 3 mL EMEM (10% FBS, 1% pen/strep), add to 7 mL medium in 75 cm flask | |||
Revision as of 13:49, 31 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Expanding Cells
Expand cultures 4x
|