Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29: Difference between revisions

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|-valign="top"
|-valign="top"
| <u>Reagent</u> || <u>Volume</u> || <u> Master Mix </u>
| <u>Reagent</u> || <u>Volume</u> || <u> Master Mix </u>
| rowspan="7" | <u>Expected:</u><br>1,2. KAH123 = 8002<br>3,4. KAH124 = 8174<br>5,6. KAH125 = 8246<br>
| rowspan="7" | [[Image:CA_Gel.jpeg|300px|E/P digests 8/29/12]]<br>15 μL/lane; 1% agarose -->
| rowspan="7" | [[Image:KAH_121509_gel1.tif|300px|E/P digests 12/15/09]]<br>15 μL/lane; 1% agarose -->
|-
|-
| DNA(plasmid) || 3.0 μL || -
| DNA(plasmid) || 3.0 μL || -

Revision as of 12:57, 5 September 2012

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Digestion

  • We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
  • Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126


Reagent Volume Master Mix E/P digests 8/29/12
15 μL/lane; 1% agarose -->
DNA(plasmid) 3.0 μL -
10X buffer 1.5 4.5
EcoRI 1.0 3.0
PstI 1.0 3.0
dH2O 8.5 25.5
  15 μL --> 37°C/ ~15 min.


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