Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Digestion)
Line 12: Line 12:
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->
 
|-valign="top"
 
|-valign="top"
| <u>Reagent</u> || <u>Volume</u>  
+
| <u>Reagent</u> || <u>Volume</u> || <u> Master Mix </u>
 
| rowspan="7" | <u>Expected:</u><br>1,2. KAH123 = 8002<br>3,4. KAH124 = 8174<br>5,6. KAH125 = 8246<br>
 
| rowspan="7" | <u>Expected:</u><br>1,2. KAH123 = 8002<br>3,4. KAH124 = 8174<br>5,6. KAH125 = 8246<br>
 
| rowspan="7" | [[Image:KAH_121509_gel1.tif|300px|E/P digests 12/15/09]]<br>15 μL/lane; 1% agarose -->
 
| rowspan="7" | [[Image:KAH_121509_gel1.tif|300px|E/P digests 12/15/09]]<br>15 μL/lane; 1% agarose -->

Revision as of 11:30, 5 September 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Digestion

  • We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
  • Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126


Reagent Volume Master Mix Expected:
1,2. KAH123 = 8002
3,4. KAH124 = 8174
5,6. KAH125 = 8246
File:KAH 121509 gel1.tif
15 μL/lane; 1% agarose -->
DNA(plasmid) 3.0 μL -
10X buffer 1.5 4.5
EcoRI 1.0 3.0
PstI 1.0 3.0
dH2O 8.5 25.5
  15 μL --> 37°C/ ~15 min.