Difference between revisions of "Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29"

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(Digestion)
(Digestion)
Line 8: Line 8:
 
==Digestion==
 
==Digestion==
 
* We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
 
* We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
* Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126
+
* Part: [http://partsregistry.org/wiki/index.php?title=Part:BBa_J176062 BBa_J176122], Vector: MV2, Transfection Plasmid: [http://partsregistry.org/wiki/index.php?title=Part:BBa_J176062 KAH126]
 
<br>
 
<br>
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->

Revision as of 13:12, 5 September 2012

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Digestion

  • We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
  • Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126


Reagent Volume Master Mix E/P digests 8/29/12
15 μL/lane; 1% agarose -->
KAH Fermentas GeneRuler 1kbplus.jpg
Gene Ruler -->
DNA(plasmid) 3.0 μL -
10X buffer 1.5 4.5
EcoRI 1.0 3.0
PstI 1.0 3.0
dH2O 8.5 25.5
  15 μL --> 37°C/ ~15 min.