Difference between revisions of "Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/11/09"

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==Entry title==
 
==Entry title==
* Insert content here...
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Reran gel of 9/21 experiment
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Ran time course:
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pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng
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19.2uL 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL
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topo eq. 0.5uL 0.5uL 0.5uL 0.5uL 0.5uL 1uL EcoRI
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NEB 4 3uL 3uL 3uL 3uL 3uL 3uL
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ddH20 up to 30
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total 30uL 30uL 30uL 30uL 30uL 30uL
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incubation:37C 4min 6 8 10 12 12+
  
  

Revision as of 00:04, 3 December 2013

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Entry title

Reran gel of 9/21 experiment

Ran time course: pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL topo eq. 0.5uL 0.5uL 0.5uL 0.5uL 0.5uL 1uL EcoRI

NEB 4 3uL 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 30uL 30uL 30uL 30uL 30uL 30uL incubation:37C 4min 6 8 10 12 12+