Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/09/22

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-Miniprepped TopoD168A pET29a liquid colonies -Performed Bradford assay in triplicate 1 2 3 4 5 6 A blank - - 50%gly - - B 0.1mg/mL - - 1/10topo - - C .25mg/mL - - 1/2 topo - - D .5gmg/mL - - E .75mg/mL - - F 1mg/mL - - G 2mg/mL - - H

Used 250 of 1/5 diluted Bradford, 5uL BSA standard/sample in each well