Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/10/14

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10/14/13

Good expected growth on streak plate, and good growth in both liquid cultures.

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.161 1.85 160.992
2. flyPCD 0.358 1.818 358.374

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.

Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone.

DNA Sequencing Samples

  • Submitted to DNASU on: '11/14/2013'
  • Order Number: 8388
  1. Plasmid 1 AmCyan - forward primer 'P001'
  2. Plasmid 1 AmCyan - reverse primer 'P002'
  3. Plasmid 2 flyPCD - forward primer 'P001'
  4. Plasmid 2 flyPCD - reverse primer 'P002'