Difference between revisions of "Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/09/21"

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(List of Samples)
(List of Samples)
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*'''RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again'''
  
 
'''DNA Sequencing Samples'''
 
'''DNA Sequencing Samples'''

Revision as of 14:57, 21 September 2013

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09/21/13

  • Growth on streak plate
  • AmCyan and flyPCD
    • Inoculated: 9/20/13
    • Culture 1(AmCyan): Growth
    • Culture 2(flyPCD): Growth

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.034 1.8 34.227
2. flyPCD 0.025 1.953 24.576

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.
  • RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again

DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'