Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 8: | Line 8: | ||
==Incubation in Liquid Media== | ==Incubation in Liquid Media== | ||
#Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | #Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | ||
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids. | # Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids. | ||
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube. | # Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube. | ||
# Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube. | # Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube. | ||
# Followed the same procedure with David's and Heather's culture tubes and plates. | # Followed the same procedure with David's and Heather's culture tubes and plates. | ||
# Placed all three tubes into the incubator. | # Placed all three tubes into the incubator. |
Revision as of 15:36, 27 July 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Incubation in Liquid Media
NotesNOTE: Plates were NOT wrapped in parafilm, so colonies may have been dried/dead when they were chosen Plates were placed in the silver fridge on top of the blue plastic lid. |