Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Notes) |
(fix raw html notebook nav) |
||
(12 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Incubation in Liquid Media== | ==Incubation in Liquid Media 7/27/13== | ||
#Gathered 3 culture tubes, the three plates(Prad(mine), David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | |||
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids (Prad-KAH013, David-KAH06, Heather- also KAH013). | |||
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to culture tube. | |||
# Circled the selected colony on the plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into the culture tube. | |||
# Followed the same procedure with David's and Heather's culture tubes and plates. | |||
# Placed all three tubes into the incubator. | |||
*Plates were wrapped in parafilm and placed inside the silver fridge. | |||
==Notes== | ==Notes== | ||
'''NOTE: Plates were NOT wrapped in parafilm, | '''NOTE: Plates were NOT wrapped in parafilm when I first saw them, and what appeared to be dry patches were on all 3 plates.''' | ||
Plates were placed in the silver fridge on top of the blue plastic lid. | Plates were placed in the silver fridge on top of the blue plastic lid. | ||
Latest revision as of 23:08, 26 September 2017
Project name | Main project page Previous entry Next entry |
Incubation in Liquid Media 7/27/13
NotesNOTE: Plates were NOT wrapped in parafilm when I first saw them, and what appeared to be dry patches were on all 3 plates. Plates were placed in the silver fridge on top of the blue plastic lid. |