Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27

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Revision as of 23:13, 2 December 2013 by Pradyumna Kadambi (talk | contribs) (Incubation in Liquid Media 7/27/13)
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Incubation in Liquid Media 7/27/13

  1. Gathered 3 culture tubes, the three plates(Prad(mine), David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
  2. Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids (Prad-KAH013, David-KAH06, Heather- also KAH013).
  3. Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to culture tube.
  4. Circled the selected colony on the plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into the culture tube.
  5. Followed the same procedure with David's and Heather's culture tubes and plates.
  6. Placed all three tubes into the incubator.
  • Plates were wrapped in parafilm and placed inside the silver fridge.


NOTE: Plates were NOT wrapped in parafilm when I first saw them, so colonies may have been dried/dead when they were chosen

Plates were placed in the silver fridge on top of the blue plastic lid.