Difference between revisions of "Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi"

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(Notes)
(Notes)
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==Notes==
 
==Notes==
* Transformation of DH5
+
* Transformation of DH5 was successful. Colonies were growing in agar+amp gel.
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25 6/25/13 for protocol]
+
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25 6/25/13]
  
* Example: This project is currently on hold until further notice.
+
*Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.
 +
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/28#Spectrophotometer_analysis 6/28/13]
  
  

Revision as of 20:23, 2 July 2013

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Project Description

This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful.

Notes

  • Transformation of DH5 was successful. Colonies were growing in agar+amp gel.

6/25/13

  • Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.

6/28/13


Recent changes

26 September 2017

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User:Merman‎‎ (2 changes | history) . . (+32). . [Merman‎ (2×)]

     

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Hoatlin:BMB seminar series 2017-2018‎‎ (2 changes | history) . . (+24). . [Maureen E. Hoatlin‎ (2×)]

     

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     07:11 (Move log) . . Karmella Haynes (talk | contribs) moved page BME100 f2017:Group6 W1030 L6 to BME100 f2017:Group6 W1030 L6 delete this page(Class assignment. Students did not follow instructions for generating a page with correct formatting. Freeing up the page title to allow students to re-generate the correct page.)
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Butlin:Unix for Bioinformatics - advanced tutorial‎‎ (3 changes | history) . . (+334). . [Claudius E Kerth‎ (3×)]

     

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25 September 2017

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BME100 f2017:Group12 W0800 L3‎‎ (12 changes | history) . . (+3,361). . [Priscilla Y. Han‎ (12×)]

     

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(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

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BME100 f2017:Group8 W1030 L3‎‎ (9 changes | history) . . (+501). . [Jennifer L. Brodsky‎ (9×)]

     

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(cur | prev) . . (+5). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

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N    20:17  BME100 f2017:Group10 W0800 L3‎ (diff | hist) . . (+767). . Whitney Hirano (talk | contribs) (Created page with "5. The mean value of heart rate calculated from the pulse ox was 98.3673 beats/min with a standard deviation of 22.2273, while the mean value of heart rate calculated from the...")
     19:56  BME100 f2017:Group9 W0800 L3‎ (diff | hist) . . (+3). . Irene Zhang (talk | contribs) (LAB 3 WRITE-UP)
     19:52  BME100 f2017:Projects1‎ (diff | hist) . . (0). . Yar (talk | contribs) (small change to force cache purge)