Difference between revisions of "Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi"

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(Autocreated Lab Notebook name=Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi, content from MediaWiki:ProjectContentDefault)
 
(Project Description/Abstract)
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==Project Description/Abstract==
+
==Pradyumna Kadambi/Undergraduate Lab Traning==
* Place short description of project or notes regarding this project
+
 
 +
'''DH5α Transformation with KAH013 Plasmid 6/25/2013'''<br>
 +
#In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
 +
#Waited 10 minutes for bacteria to accept plasmid.
 +
#Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
 +
#Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
 +
#Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).
 +
 
 +
'''DH5α Incubation in Liquid Media 6/28/2013'''<br>
 +
#With a pipetter, 3mL of LB+amp was added to a test tube.
 +
#A colony was selected on the culture plate(plate was overgrown), and a circle was drawn where the colony was selected.
 +
#Micropipette tip was lightly touched to the colony making sure not to scrape any of the agar gel. Micropipette tip was then ejected into test tube.
 +
#Test tube was incubated with shaker for 7 hours at 37 degrees.
 +
 
 +
''''DH5α Mini Prep with KAH013 6/28/2013'''<br>
 +
*Colonies that Rene had incubated in liquid media on 6/27 were used.
 +
#Using a micropipette, a 2mL centrifuge tube was filled with the incubated liquid media.
 +
#The solution was centrifuged at 13.1g for 3 minutes (If a pellet has not completely condensed centrifuge again until pellet does condense).
 +
#Using a micropipette, the supernatant was removed until 600μL of solution remained in the centrifuge tube. (Discard supernatant into the bleach container in the fume hood)
 +
#Pellet was broken up using the vortex.
 +
#Added 100μL of lysis buffer to the centrifuge tube and inverted 4-6 times and waited 1 min.
 +
#Added 350μL of lysis buffer. Invert the solution until it turns yellow, make sure no blue pockets are left.
 +
#Centrifuge the solution at
 +
#
 +
#
 +
#
 +
'''
 +
 
  
 
* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
 
* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.

Revision as of 18:46, 29 June 2013

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Pradyumna Kadambi/Undergraduate Lab Traning

DH5α Transformation with KAH013 Plasmid 6/25/2013

  1. In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
  2. Waited 10 minutes for bacteria to accept plasmid.
  3. Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
  4. Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
  5. Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).

DH5α Incubation in Liquid Media 6/28/2013

  1. With a pipetter, 3mL of LB+amp was added to a test tube.
  2. A colony was selected on the culture plate(plate was overgrown), and a circle was drawn where the colony was selected.
  3. Micropipette tip was lightly touched to the colony making sure not to scrape any of the agar gel. Micropipette tip was then ejected into test tube.
  4. Test tube was incubated with shaker for 7 hours at 37 degrees.

'DH5α Mini Prep with KAH013 6/28/2013

  • Colonies that Rene had incubated in liquid media on 6/27 were used.
  1. Using a micropipette, a 2mL centrifuge tube was filled with the incubated liquid media.
  2. The solution was centrifuged at 13.1g for 3 minutes (If a pellet has not completely condensed centrifuge again until pellet does condense).
  3. Using a micropipette, the supernatant was removed until 600μL of solution remained in the centrifuge tube. (Discard supernatant into the bleach container in the fume hood)
  4. Pellet was broken up using the vortex.
  5. Added 100μL of lysis buffer to the centrifuge tube and inverted 4-6 times and waited 1 min.
  6. Added 350μL of lysis buffer. Invert the solution until it turns yellow, make sure no blue pockets are left.
  7. Centrifuge the solution at


  • Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.

Notes

  • Place some notes here for visitors
  • Example: This project is currently on hold until further notice.


Recent changes

26 September 2017

     07:32 

Tomlinson:News‎‎ (2 changes | history) . . (+83). . [Ryan E. Tomlinson‎ (2×)]

     

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Tomlinson:Lab Members‎‎ (3 changes | history) . . (+46). . [Ryan E. Tomlinson‎ (3×)]

     

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(cur | prev) . . (+4). . Ryan E. Tomlinson (talk | contribs) (Kimberly Huang)

     

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     07:11 (Move log) . . Karmella Haynes (talk | contribs) moved page BME100 f2017:Group6 W1030 L6 to BME100 f2017:Group6 W1030 L6 delete this page(Class assignment. Students did not follow instructions for generating a page with correct formatting. Freeing up the page title to allow students to re-generate the correct page.)
     07:07  BME100 f2017:Projects6‎ (diff | hist) . . (+19). . Karmella Haynes (talk | contribs)
     07:07  BME100 f2017:Projects5‎ (diff | hist) . . (+19). . Karmella Haynes (talk | contribs)
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     07:01  BME100 f2017:Projects3‎ (diff | hist) . . (+19). . Karmella Haynes (talk | contribs)
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     04:06 

Butlin:Unix for Bioinformatics - advanced tutorial‎‎ (3 changes | history) . . (+334). . [Claudius E Kerth‎ (3×)]

     

04:06

(cur | prev) . . (+148). . Claudius E Kerth (talk | contribs) (Added troublehooting tip: when rename.pl download link does not work, go to my Dropbox)

     

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25 September 2017

     21:19 

BME100 f2017:Group12 W0800 L3‎‎ (12 changes | history) . . (+3,361). . [Priscilla Y. Han‎ (12×)]

     

21:19

(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

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     21:19 

(Upload log). .

[Priscilla Y. Han‎ (7×)]

     

21:19

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     20:33 

BME100 f2017:Group8 W1030 L3‎‎ (9 changes | history) . . (+501). . [Jennifer L. Brodsky‎ (9×)]

     

20:33

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N    20:17  BME100 f2017:Group10 W0800 L3‎ (diff | hist) . . (+767). . Whitney Hirano (talk | contribs) (Created page with "5. The mean value of heart rate calculated from the pulse ox was 98.3673 beats/min with a standard deviation of 22.2273, while the mean value of heart rate calculated from the...")
     19:56  BME100 f2017:Group9 W0800 L3‎ (diff | hist) . . (+3). . Irene Zhang (talk | contribs) (LAB 3 WRITE-UP)

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