Haynes Lab:Notebook/Jan/2013/10/16

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Growing Liquid Culture of mCh

  • mCh / BBa_J176005
    • Transformation done on 10/15/13
    • Result for Plate 1: Good growth over much of the plate. Overall success.
    • Result for Plate 2: Growth over about half of the plate, indicating an error in procedure.

Because there was growth on the negative control plate, indicating a fault in the transformation, the plates were disposed of and the transformation was redone.

  • Plasmid name: mCh in V0120 vector
    • BioBrick name: BBa_J176005 BBa_J176005
    • Vector name: V0120 (BBa_J176127)
    • Ampicillin resistance on V0120
    • Length of vector: 3200 bp
  • Sample 1: Plasmid DNA - mCh (BBa_J176005)
  • Sample 2: negative control - water (Vol 1, p. 166)