|Project name||<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page|
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
Plasmid Extraction and Validation of fshPCD
DNA Concentration Data
Conclusion: All samples had a band just under the 5000 bp band (the brightest top band), which was probably the 3200 bp vector. Also, the expected part base pair was 186 bp, and the band in the picture is slightly above the 200 bp band, but all three samples including the control DNA had the band at the same location, so overall, the restriction digest was successful. However, additional help about the two bands around the 1500 bp location that appeared in samples 1 and 2 but not in the control DNA will be received before continuing. After talking with the professor, a possible explanation is that this band was supercoiled undigested plasmid DNA. It was decided to submit samples 1 and the control DNA for DNA sequencing.
DNA Sequencing Samples