Haynes Lab:Notebook/Jan/2013/09/27
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Plasmid Extraction and Validation of fshPCD
DNA Concentration Data
Conclusion: All samples had a band just under the 5000 bp band (the brightest top band), which was probably the 4986 bp vector. Also, the expected part base pair was 186 bp, and the band in the picture is slightly above the 200 bp band, but all three samples including the control DNA had the band at the same location, so overall, the restriction digest was successful. However, additional help about the two bands around the 1500 bp location that appeared in samples 1 and 2 but not in the control DNA will be received before continuing. DNA Sequencing Samples
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