Difference between revisions of "Haynes Lab:Notebook/Jan/2013/09/25"

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==Entry title==
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==Plasmid Transformation==
 
* Plasmid name: fshPCD in V0120 vector
 
* Plasmid name: fshPCD in V0120 vector
 
** BioBrick name: BBa_J176002 [http://parts.igem.org/Part:BBa_J176002 BBa_J176002]
 
** BioBrick name: BBa_J176002 [http://parts.igem.org/Part:BBa_J176002 BBa_J176002]
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* Sample 2: negative control - water (Vol 1, p. 166)
 
* Sample 2: negative control - water (Vol 1, p. 166)
  
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* Sample 1 had moderate growth from ~6:30 pm to ~10:30 am--transformation was successful.
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* Sample 2 had no growth save for a couple of colonies. Overall the transformation was successful.
  
  

Revision as of 09:51, 26 September 2013

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Plasmid Transformation

  • Plasmid name: fshPCD in V0120 vector
    • BioBrick name: BBa_J176002 BBa_J176002
    • Vector name: V0120 (BBa_J176127)
    • Ampicillin resistance on V0120
    • Length of vector: 4968 bp
  • Sample 1: Plasmid DNA - fshPCD (BBa_J176002)
  • Sample 2: negative control - water (Vol 1, p. 166)
  • Sample 1 had moderate growth from ~6:30 pm to ~10:30 am--transformation was successful.
  • Sample 2 had no growth save for a couple of colonies. Overall the transformation was successful.