Difference between revisions of "Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/05/02"

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(May 2, 2013)
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* Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
 
* Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
  
[[Image:VN_Mutagenesis_Transformation_Trial_2_DH5a.png‎|thumb|frame|center|The next day, no colonies were seen for the H2B mutagenesis but there were about 25 colonies for the LOV mutagenesis.]]
+
[[Image:VN_Mutagenesis_Transformation_Trial_2_DH5a.png‎|thumb|frame|center|The next day, no colonies were seen for the H2B mutagenesis but there were about 20 colonies for the LOV mutagenesis.]]
  
  

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May 2, 2013

  • Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
The next day, no colonies were seen for the H2B mutagenesis but there were about 20 colonies for the LOV mutagenesis.