Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| Gibson master mix || 15.0 || 15.0 || 15.0 | | Gibson master mix || 15.0 || 15.0 || 15.0 | ||
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| Total volume || 20.0 || 20.0 || 18.0 | |||
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| colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. | | colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. | ||
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Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | ||
[[Image:VN_Gibson_Assembly_Trial_1.png|thumb|frame|center|No colonies were seen the next day.]] | |||
'''Made more pSB1A3 stock''' | |||
{| class="wikitable" border=1 cellpadding="5" cellspacing="0" | |||
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|Trial || 260 || 280 || 260/280 || ng/μL | |||
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|1 || 0.062|| 0.035 || 1.773|| 62.46 | |||
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|2 || 0.052|| 0.03 || 1.722|| 52.032 | |||
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|3 || 0.082|| 0.047|| 1.762|| 82.222 | |||
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Latest revision as of 22:19, 26 September 2017
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December 6, 2012Assemblies
Dephosphorylation (11/23/12) > Dephosphorylation (Roche)
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 Made more pSB1A3 stock
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