Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/23: Difference between revisions
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Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | ||
[[Image:VN_H2B_and_LOV_Transformation_Trial_2.png|thumb|frame|center|There were no cultures seen the next day.]] | |||
'''Digestion''' | '''Digestion''' |
Revision as of 07:18, 8 December 2012
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November 23, 2012Dephosphorylation (11/23/12) > Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 Digestion
Results of the enzyme digests are shown, with four H2B bands on the left, and three LOV bands on the right. The gel was loaded with 30 μL samples. The bands are at 2000 bp, confirming the vector self ligated and there is no insert.
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