Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==November 12, 2012== | ==November 12, 2012== | ||
* The PCR products were purified with the Zymo DNA Clean | * The PCR products were purified with the Zymo DNA Clean & Concentrator Kit | ||
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** In a 30 μL reaction, cut H2B with XbaI and SpeI | ** In a 30 μL reaction, cut H2B with XbaI and SpeI | ||
** In a 30 μL reaction, cut LOV with XbaI and SpeI | ** In a 30 μL reaction, cut LOV with XbaI and SpeI | ||
** In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI | ** In a 30 μL reaction, cut a [http://partsregistry.org/Part:pSB1A2 pSB1A2 plasmid] (from Rene) XbaI and SpeI | ||
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Latest revision as of 22:14, 26 September 2017
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November 12, 2012
Great! Next step...
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