Difference between revisions of "Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06"

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(November 6, 2012)
(November 6, 2012)
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[[Image:VN_Gel_11-6-12.png|100px|Results of the digests are shown.]]
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[[Image:VN_Gel_11-6-12.png|150px|Results of the digests are shown.]]
 
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]
 
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]
 
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Revision as of 12:43, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/PstI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 0
PstI 0 1.0 1.0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0


Expected:
1. H2B-GFP/ EcoRI = 4885, 210
2. H2B-GFP/ PstI = 5101
3. LOV/PstI = 251

Results of the digests are shown. KAH Fermentas GeneRuler 1kbplus.jpg