Difference between revisions of "Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06"

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(November 6, 2012)
(November 6, 2012)
Line 17: Line 17:
 
  ! scope="col" style="background:#efefef;" | LOV/EcoRI
 
  ! scope="col" style="background:#efefef;" | LOV/EcoRI
 
  |-
 
  |-
  |DNA(plasmid)  |2.0  |2.0  |2.0
+
  |DNA(plasmid)
 +
  |2.0
 +
  |2.0
 +
  |2.0
 
  |-
 
  |-
 
  |10x buffer
 
  |10x buffer

Revision as of 06:43, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/EcoRI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 1.0
PstI 0 1.0 0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0

[[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]