Difference between revisions of "Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06"

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(Entry title)
(November 6, 2012)
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  |EcoRI
 
  |EcoRI
 
  |1
 
  |1
  |---
+
  |0
 
  |1
 
  |1
 
  |-
 
  |-
 
  |PstI
 
  |PstI
  |---
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  |0
 
  |1
 
  |1
  |---
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  |0
 
  |dH<sub>2</sub>O
 
  |dH<sub>2</sub>O
 
  |10.5
 
  |10.5

Revision as of 06:38, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/EcoRI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1 0 1
PstI 0 1 0 dH2O 10.5 10.5 10.5
Total 15 15 15

[[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]