Haynes Lab:Notebook/Inteins/2013/03/01

From OpenWetWare
Revision as of 14:58, 5 March 2013 by Amanda Ispas (talk | contribs) (Entry title)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Completed a restriction/digest from really good mini prep!
  • Used brand new enzymes for the restriction/ digest
  • Made a brand new gel

1KBT KAH9(186) KAH9(160) KAH10 (116) KAH10 (106)

  • Put 10 of ladder, 30 in other wells.
  • Run dna recovery kit
    • Melted @ 55.6 degrees C for 6 min., vortex in between
    • 8uL + 7 water to elute dna in last step
  • Completed ligation/transformation
  • Used both 2x and 10x buffers
  • Get competent cells and keep on ice
  • Used both buffers to see if lab shared stock Roche ligation buffer was contaminated.
  • Used brand new ligase and ligation buffer
  • Left ligations for at least 30 mins.
  • Add 50 uL of competent cells