Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/03/01"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
 
==Entry title==
* Insert content here...
+
* Completed a restriction/digest from really good mini prep!
 +
*Used brand new enzymes for the restriction/ digest
 +
*Made a brand new gel
 +
1KBT  KAH9(186)  KAH9(160)  KAH10 (116)  KAH10 (106) 
 +
*Put 10 of ladder, 30 in other wells.
 +
http://openwetware.org/wiki/Image:3-1-2013_gel.jpg
 +
*Run dna recovery kit
 +
**Melted @ 55.6 degrees C for 6 min., vortex in between
 +
**8uL + 7 water to elute dna in last step
 +
 
 +
*Completed ligation/transformation
 +
*Used both 2x and 10x buffers
 +
*Get competent cells and keep on ice
 +
*Used both buffers to see if lab shared stock Roche ligation buffer was contaminated.
 +
*Used brand new ligase and ligation buffer
 +
*Left ligations for at least 30 mins.
 +
*Add 50 uL of competent cells
 +
 
  
  

Latest revision as of 21:31, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

  • Completed a restriction/digest from really good mini prep!
  • Used brand new enzymes for the restriction/ digest
  • Made a brand new gel

1KBT KAH9(186) KAH9(160) KAH10 (116) KAH10 (106)

  • Put 10 of ladder, 30 in other wells.

http://openwetware.org/wiki/Image:3-1-2013_gel.jpg

  • Run dna recovery kit
    • Melted @ 55.6 degrees C for 6 min., vortex in between
    • 8uL + 7 water to elute dna in last step
  • Completed ligation/transformation
  • Used both 2x and 10x buffers
  • Get competent cells and keep on ice
  • Used both buffers to see if lab shared stock Roche ligation buffer was contaminated.
  • Used brand new ligase and ligation buffer
  • Left ligations for at least 30 mins.
  • Add 50 uL of competent cells