Haynes Lab:Notebook/Inteins/2013/02/19

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Entry title

  • Completed a restriction/digest
  • Incubated @ 37.5- 37 degrees C for 20 mins.
  • Completed a gel
  • 1KBT 9(186) 9(160) 10(116) 10(106)
  • 8.5 of the ladder, 30 uL for dna wells
  • Also did a gel extraction
    • 300 uL ADB Buffer of every gel slice
    • Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
    • Follow the gel dna recovery kit