Haynes Lab:Notebook/Inteins/2013/02/19: Difference between revisions

Latest revision as of 22:31, 26 September 2017

Project name

Completed a restriction/digest
Incubated @ 37.5- 37 degrees C for 20 mins.
Completed a gel
1KBT 9(186) 9(160) 10(116) 10(106)
8.5 of the ladder, 30 uL for dna wells
Also did a gel extraction
- 300 uL ADB Buffer of every gel slice
- Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
- Follow the gel dna recovery kit

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