Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/02/19"

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(Autocreate 2013/02/19 Entry for Haynes_Lab:Notebook/Inteins)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
 
==Entry title==
* Insert content here...
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* Completed a restriction/digest
 
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*Incubated @ 37.5- 37 degrees C for 20 mins.
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*Completed a gel
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*1KBT  9(186)  9(160)  10(116)  10(106)
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*8.5 of the ladder, 30 uL for dna wells
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*Also did a gel extraction
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**300 uL ADB Buffer of every gel slice
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**Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
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**Follow the gel dna recovery kit
  
 
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Latest revision as of 21:31, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

  • Completed a restriction/digest
  • Incubated @ 37.5- 37 degrees C for 20 mins.
  • Completed a gel
  • 1KBT 9(186) 9(160) 10(116) 10(106)
  • 8.5 of the ladder, 30 uL for dna wells
  • Also did a gel extraction
    • 300 uL ADB Buffer of every gel slice
    • Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
    • Follow the gel dna recovery kit