Haynes Lab:Notebook/Inteins/2013/02/13: Difference between revisions
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==Entry title== | ==Entry title== | ||
*Redid the restriction/digest on 9/10 | |||
*Incubated for 20 mins. | |||
*Added 10 ul of the ladder, and 30 ul of the dna | |||
*Ran gel for more than 30 mins to run gel | |||
*KAH 9 is 687 bp | |||
*KAH 10 is NLS | |||
*Vo120 is 2400 bp for backbone | |||
*Cut out 10B for extraction | |||
*Do another restriction/digest for longer with the enzymes | |||
* http://openwetware.org/wiki/Image:2013-02-13_gel.jpg | * http://openwetware.org/wiki/Image:2013-02-13_gel.jpg | ||
Revision as of 11:23, 5 March 2013
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